Brena, DavidBertran, JoanPorta de la Riva, MontserratGuillén, YolandaCornes Maragliano, Eric, 1987-Kukhtar, DmytroCampos-Vicens, LluísFernández, LierniPecharroman, IreneGarcía-López, AlbertIslam, Abul, 1978-Marruecos, LauraBigas Salvans, AnnaCerón Madrigal, JuliánEspinosa Blay, Lluís2022-02-112022-02-112020Brena D, Bertran J, Porta-de-la-Riva M, Guillén Y, Cornes E, Kukhtar D, et al. Ancestral function of Inhibitors-of-kappaB regulates Caenorhabditis elegans development. Sci Rep. 2020 Sep 30; 10(1): 16153. DOI: 10.1038/s41598-020-73146-52045-2322http://hdl.handle.net/10230/52473Mammalian IκB proteins (IκBs) exert their main function as negative regulators of NF-κB, a central signaling pathway controlling immunity and inflammation. An alternative chromatin role for IκBs has been shown to affect stemness and cell differentiation. However, the involvement of NF-κB in this function has not been excluded. NFKI-1 and IKB-1 are IκB homologs in Caenorhabditis elegans, which lacks NF-κB nuclear effectors. We found that nfki-1 and ikb-1 mutants display developmental defects that phenocopy mutations in Polycomb and UTX-1 histone demethylase, suggesting a role for C. elegans IκBs in chromatin regulation. Further supporting this possibility (1) we detected NFKI-1 in the nucleus of cells; (2) NFKI-1 and IKB-1 bind to histones and Polycomb proteins, (3) and associate with chromatin in vivo, and (4) mutations in nfki-1 and ikb-1 alter chromatin marks. Based on these results, we propose that ancestral IκB inhibitors modulate Polycomb activity at specific gene subsets with an impact on development.application/pdfengCopyright © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Caenorhabditis elegansCromatinaGenèticaMetabolisme--Aspectes genèticsinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-020-73146-5info:eu-repo/semantics/openAccess