Baquero Pérez, BelindaBortoletto, EnricoRosani, UmbertoDelgado-Tejedor, AnnaMedina, RebecaNovoa, Eva MariaVenier, PaolaDíez Antón, Juana, 1962-2024-09-182024-09-182024Baquero-Pérez B, Bortoletto E, Rosani U, Delgado-Tejedor A, Medina R, Novoa EM, et al. Elucidation of the epitranscriptomic RNA modification landscape of Chikungunya Virus. Viruses. 2024 Jun 12;16(6):945. DOI: 10.3390/v160609451999-4915http://hdl.handle.net/10230/61147The genomes of positive-sense (+) single-stranded RNA (ssRNA) viruses are believed to be subjected to a wide range of RNA modifications. In this study, we focused on the chikungunya virus (CHIKV) as a model (+) ssRNA virus to study the landscape of viral RNA modification in infected human cells. Among the 32 distinct RNA modifications analysed by mass spectrometry, inosine was found enriched in the genomic CHIKV RNA. However, orthogonal validation by Illumina RNA-seq analyses did not identify any inosine modification along the CHIKV RNA genome. Moreover, CHIKV infection did not alter the expression of ADAR1 isoforms, the enzymes that catalyse the adenosine to inosine conversion. Together, this study highlights the importance of a multidisciplinary approach to assess the presence of RNA modifications in viral RNA genomes.application/pdfeng© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/v16060945ADAR1RNA modificationsAlphavirusesChikungunya virusEpitranscriptomeInosineinfo:eu-repo/semantics/openAccess