Blevins, William Robert, 1987-Ruiz Orera, Jorge, 1988-Messeguer, XavierBlasco Moreno, Bernat, 1986-Villanueva Cañas, José Luis, 1984-Espinar, LorenaDíez Antón, Juana, 1962-Carey, Lucas, 1980-Albà Soler, Mar2021-02-162021-02-162021Blevins WR, Ruiz-Orera J, Messeguer X, Blasco-Moreno B, Villanueva-Cañas JL, Espinar L, Díez J, Carey LB, Albà MM. Uncovering de novo gene birth in yeast using deep transcriptomics. Nat Commun. 2021; 12(1):604. DOI: 10.1038/s41467-021-20911-32041-1723http://hdl.handle.net/10230/46484De novo gene origination has been recently established as an important mechanism for the formation of new genes. In organisms with a large genome, intergenic and intronic regions provide plenty of raw material for new transcriptional events to occur, but little is know about how de novo transcripts originate in more densely-packed genomes. Here, we identify 213 de novo originated transcripts in Saccharomyces cerevisiae using deep transcriptomics and genomic synteny information from multiple yeast species grown in two different conditions. We find that about half of the de novo transcripts are expressed from regions which already harbor other genes in the opposite orientation; these transcripts show similar expression changes in response to stress as their overlapping counterparts, and some appear to translate small proteins. Thus, a large fraction of de novo genes in yeast are likely to co-evolve with already existing genes.application/pdfeng© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41467-021-20911-3EvolvabilityFungal geneticsMolecular evolutionTranscriptomicsinfo:eu-repo/semantics/openAccess