Escobar-Niño, AlmudenaLiñeiro, EvaAmil, FranciscoCarrasco, Raphaël, 1950-Chiva, CristinaFuentes, CarlosBlanco-Ulate, BarbaraCantoral Fernández, Jesús M.Sabidó Aguadé, Eduard, 1981-Fernández-Acero, Francisco Javier2019-10-012019-10-012019Escobar-Niño A, Liñeiro E, Amil F, Carrasco R, Chiva C, Fuentes C et al. Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation. Scientific Reports. 2019;9(1):9860. DOI: 10.1038/s41598-019-46270-02045-2322http://hdl.handle.net/10230/42364Protein phosphorylation and membrane proteins play an important role in the infection of plants by phytopathogenic fungi, given their involvement in signal transduction cascades. Botrytis cinerea is a well-studied necrotrophic fungus taken as a model organism in fungal plant pathology, given its broad host range and adverse economic impact. To elucidate relevant events during infection, several proteomics analyses have been performed in B. cinerea, but they cover only 10% of the total proteins predicted in the genome database of this fungus. To increase coverage, we analysed by LC-MS/MS the first-reported overlapped proteome in phytopathogenic fungi, the "phosphomembranome" of B. cinerea, combining the two most important signal transduction subproteomes. Of the 1112 membrane-associated phosphoproteins identified, 64 and 243 were classified as exclusively identified or overexpressed under glucose and deproteinized tomato cell wall conditions, respectively. Seven proteins were found under both conditions, but these presented a specific phosphorylation pattern, so they were considered as exclusively identified or overexpressed proteins. From bioinformatics analysis, those differences in the membrane-associated phosphoproteins composition were associated with various processes, including pyruvate metabolism, unfolded protein response, oxidative stress response, autophagy and cell death. Our results suggest these proteins play a significant role in the B. cinerea pathogenic cycle.application/pdfeng© The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-019-46270-0Fungal pathogenesisProtein–protein interaction networksinfo:eu-repo/semantics/openAccess