Marmolejo-Martínez-Artesero, SaraRomeo-Guitart, DavidMañas García, Laura, 1991-Barreiro Portela, EstherCasas, Caty2020-10-072020-10-072020Marmolejo-Martínez-Artesero S, Romeo-Guitart D, Mañas-García L, Barreiro E, Casas C. NeuroHeal reduces muscle atrophy and modulates associated autophagy. Cells. 2020 Jun 28; 9(7):1575. DOI: 10.3390/cells90715752073-4409http://hdl.handle.net/10230/45413Muscle wasting is an unmet medical need which leads to a reduction of myofiber diameter and a negative impact on the functional performance of daily activities. We previously found that a new neuroprotective drug called NeuroHeal reduced muscle atrophy produced by transient denervation. Aiming to decipher whether NeuroHeal has a direct role in muscle biology, we used herein different models of muscle atrophy: one caused by chronic denervation, another caused by hindlimb immobilization, and lastly, an in vitro model of myotube atrophy with Tumor Necrosis Factor-α (TNFα). In all these models, we observed that NeuroHeal reduced muscle atrophy and that SIRT1 activation seems to be required for that. The treatment downregulated some critical markers of protein degradation: Muscle Ring Finger 1 (MuRF1), K48 poly-Ub chains, and p62/SQSTM1. Moreover, it seems to restore the autophagy flux associated with denervation. Hence, we envisage a prospective use of NeuroHeal at clinics for different myopathies.application/pdfengcopyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/cells9071575NeuroHealAutophagyProteasomeSirtuin 1Skeletal muscle atrophyinfo:eu-repo/semantics/openAccess