Meunier, SylvainTimón Pérez, Krystal, 1987-Vernos, Isabelle, 1959-2017-04-192017-04-192016Meunier S, Timón Pérez K, Vernos I. Aurora-A regulates MCRS1 function during mitosis. Cell Cycle. 2016;15(13):1779-86. DOI: 10.1080/15384101.2016.11873421538-4101http://hdl.handle.net/10230/30833The mitotic spindle is made of microtubules (MTs) nucleated through different pathways involving the centrosomes, the chromosomes or the walls of pre-existing MTs. MCRS1 is a RanGTP target that specifically associates with the chromosome-driven MTs protecting them from MT depolymerases. MCRS1 is also needed for the control of kinetochore fiber (K-fiber) MT minus-ends dynamics in metaphase. Here, we investigated the regulation of MCRS1 activity in M-phase. We show that MCRS1 is phosphorylated by the Aurora-A kinase in mitosis on Ser35/36. Although this phosphorylation has no role on MCRS1 localization to chromosomal MTs and K-fiber minus-ends, we show that it regulates MCRS1 activity in mitosis. We conclude that Aurora-A activity is particularly important in the tuning of K-fiber minus-ends dynamics in mitosis.application/pdfeng© 2016 The Author(s). Published with license by Taylor & Francis.© Sylvain Meunier, Krystal Timon, and Isabelle Vernos. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/15384101.2016.1187342Aurora-A kinaseChromosomal microtubulesK-fiberMCRS1Microtubule minus-endMitosisPhosphorylationinfo:eu-repo/semantics/openAccess