Fernández-Castillejo, SaraPedret, AnnaCatalán, ÚrsulaValls, Rosa M.Farràs Mañé, MartaRubió, LauraCastañer, OlgaMacià, AlbaFitó Colomer, MontserratMotilva, Maria-JoséCovas Planells, Maria IsabelGiera, MartinRemaley, Alan T.Solà, Rosa2021-10-222021-10-222021Fernández-Castillejo S, Pedret A, Catalán Ú, Valls RM, Farràs M, Rubió L, Castañer O, Macià A, Fitó M, Motilva MJ, Covas MI, Giera M, Remaley AT, Solà R. Virgin olive oil phenolic compounds modulate the HDL lipidome in hypercholesterolaemic subjects: a lipidomic analysis of the VOHF study. Mol Nutr Food Res. 2021;65(9):e2001192. DOI: 10.1002/mnfr.2020011921613-4125http://hdl.handle.net/10230/48754Scope: The lipidomic analysis of high-density lipoprotein (HDL) could be useful to identify new biomarkers of HDL function. Methods and results: A randomized, controlled, double-blind, crossover trial (33 hypercholesterolaemic subjects) is performed with a control virgin olive oil (VOO), VOO enriched with its own phenolic compounds (FVOO), or VOO enriched with additional phenolic compounds from thyme (FVOOT) for 3 weeks. HDL lipidomic analyses are performed using the Lipidyzer platform. VOO and FVOO intake increase monounsaturated-fatty acids (FAs) and decrease saturated and polyunsaturated FAs in triacylglyceride (TAG) species, among others species. In contrast, FVOOT intake does not induce these FAs changes. The decrease in TAG52:3(FA16:0) after VOO intake and the decrease in TAG52:5(FA18:2) after FVOO intake are inversely associated with changes in HDL resistance to oxidation. After FVOO intake, the decrease in TAG54:6(FA18:2) in HDL is inversely associated with changes in HDL cholesterol efflux capacity. Conclusion: VOO and FVOO consumption has an impact on the HDL lipidome, in particular TAG species. Although TAGs are minor components of HDL mass, the observed changes in TAG modulated HDL functionality towards a cardioprotective mode. The assessment of the HDL lipidome is a valuable approach to identify and characterize new biomarkers of HDL function.application/pdfeng© 2021 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/mnfr.202001192HDL functionalityCholesterol effluxLipidomicsPolyphenolsResistance to oxidationinfo:eu-repo/semantics/openAccess