Saus Martínez, EsterWillis, Jesse R.Pryszcz, Leszek Piotr, 1985-Hafez Khafaga, Ahmed Ibrahem, 1987-Llorens, Carlos, 1968-Himmelbauer, HeinzGabaldón Estevan, Juan Antonio, 1973-2019-12-052019-12-052018Saus E, Willis JR, Pryszcz LP, Hafez A, Llorens C, Himmelbauer H et al. nextPARS: parallel probing of RNA structures in Illumina. RNA. 2018;24(4):609-19. DOI: 10.1261/rna.063073.1171355-8382http://hdl.handle.net/10230/43102RNA molecules play important roles in virtually every cellular process. These functions are often mediated through the adoption of specific structures that enable RNAs to interact with other molecules. Thus, determining the secondary structures of RNAs is central to understanding their function and evolution. In recent years several sequencing-based approaches have been developed that allow probing structural features of thousands of RNA molecules present in a sample. Here, we describe nextPARS, a novel Illumina-based implementation of in vitro parallel probing of RNA structures. Our approach achieves comparable accuracy to previous implementations, while enabling higher throughput and sample multiplexing.application/pdfeng© 2018 Saus et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1261/rna.063073.117RNA secondary structureGenome-wide enzymatic probingMultiplex sequencinginfo:eu-repo/semantics/openAccess