Millanes Romero, Alba, 1986-Herranz Martín, NicolásPerrera, ValentinaIturbide Martínez de Albéniz, Ane, 1989-Loubat Casanovas, Jordina, 1982-Jenuwein, ThomasGarcía de Herreros, AntonioPeiró Sales, Sandra2015-12-012015-12-012013Millanes-Romero A, Herranz N, Perrera V, Iturbide A, Loubat-Casanovas J, Gil J et al. Regulation of heterochromatin transcription by Snail1/LOXL2 during epithelial-to-mesenchymal transition. Molecular cell. 2013;52(5):746-57. DOI: 10.1016/j.molcel.2013.10.0151097-2765http://hdl.handle.net/10230/25287Although heterochromatin is enriched with repressive traits, it is also actively transcribed, giving rise to large amounts of noncoding RNAs. Although these RNAs are responsible for the formation and maintenance of heterochromatin, little is known about how their transcription is regulated. Here, we show that the Snail1 transcription factor represses mouse pericentromeric transcription, acting through the H3K4 deaminase LOXL2. Since Snail1 plays a key role in the epithelial-to-mesenchymal transition (EMT), we analyzed the regulation of heterochromatin transcription in this process. At the onset of EMT, one of the major structural heterochromatin proteins, HP1α, is transiently released from heterochromatin foci in a Snail1/LOXL2-dependent manner, concomitantly with a downregulation of major satellite transcription. Moreover, preventing the downregulation of major satellite transcripts compromised the migratory and invasive behavior of mesenchymal cells. We propose that Snail1 regulates heterochromatin transcription through LOXL2, thus creating the favorable transcriptional state necessary for completing EMT.application/pdfeng© Elsevier. This is the published version of an article http://10.1016/j.molcel.2013.10.015 that appeared in the journal Molecular cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseProteïnesFactors de transcripcióHeterocromatinaRegulation of heterochromatin transcription by Snail1/LOXL2 during epithelial-to-mesenchymal transitioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.molcel.2013.10.015info:eu-repo/semantics/openAccess