Carrera-Aubesart, AdamDefaus, SiraPérez-Peinado, Clara, 1991-Sandín, DanielTorrent Burgas, MarcJiménez, M. ÁngelesAndreu Martínez, David2022-11-152022-11-152022Carrera-Aubesart A, Defaus S, Pérez-Peinado C, Sandín D, Torrent M, Jiménez MÁ, Andreu D. Examining topoisomers of a snake-venom-derived peptide for improved antimicrobial and antitumoral properties. Biomedicines. 2022 Aug 29;10(9):2110. DOI: 10.3390/biomedicines100921102227-9059http://hdl.handle.net/10230/54847Ctn[15-34], the C-terminal section of crotalicidin (Ctn), a cathelicidin from a South American pit viper, is an antimicrobial and antitumoral peptide with remarkably longer stability in human serum than the parent Ctn. In this work, a set of topoisomers of both Ctn and Ctn[15-34], including the retro, enantio, and retroenantio versions, were synthesized and tested to investigate the structural requirements for activity. All topoisomers were as active as the cognate sequences against Gram-negative bacteria and tumor cells while slightly more toxic towards normal cells. More importantly, the enhanced serum stability of the D-amino-acid-containing versions suggests that such topoisomers must be preferentially considered as future antimicrobial and anticancer peptide leads.application/pdfeng© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Examining topoisomers of a snake-venom-derived peptide for improved antimicrobial and antitumoral propertiesinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/biomedicines10092110Ctn[15-34]Antimicrobial peptidesAntitumoral peptidesCrotalicidinEnantio-, retro-, and retroenantio peptidesSnake venomTopoisomer peptidesinfo:eu-repo/semantics/openAccess