Niu, DongWei, Hong-JiangLin, LinGeorge, HaydyWang, TaoLee, I-HsiuZhao, Hong-YeWang, YongKan, YinanShrock, EllenLesha, EmalWang, GangLuo, YonglunQing, YuboJiao, DelingZhao, HengZhou, XiaoyangWang, ShouqiWei, Hong-JiangGüell Cargol, Marc, 1982-Church, George M.Yang, Luhan2018-11-142018-11-142017Niu D, Wei HJ, Lin L, George H, Wang T, Lee IH et al. Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9. Science. 2017 Sep 22;357(6357):1303-7. DOI: 10.1126/science.aan41870036-8075http://hdl.handle.net/10230/35752Xenotransplantation is a promising strategy to alleviate the shortage of organs for human transplantation. In addition to the concerns about pig-to-human immunological compatibility, the risk of cross-species transmission of porcine endogenous retroviruses (PERVs) has impeded the clinical application of this approach. We previously demonstrated the feasibility of inactivating PERV activity in an immortalized pig cell line. We now confirm that PERVs infect human cells, and we observe the horizontal transfer of PERVs among human cells. Using CRISPR-Cas9, we inactivated all of the PERVs in a porcine primary cell line and generated PERV-inactivated pigs via somatic cell nuclear transfer. Our study highlights the value of PERV inactivation to prevent cross-species viral transmission and demonstrates the successful production of PERV-inactivated animals to address the safety concern in clinical xenotransplantation.application/pdfengThis is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science on Vol. 357 Issue 6357, 22 Sep 2017. DOI: 10.1126/science.aan4187XenotrasplantamentMalalties transmissiblesSilenciament gènicinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1126/science.aan4187info:eu-repo/semantics/openAccess