Guillaumet-Adkins, AmyRodríguez Esteban, GustavoMereu, ElisabettaMéndez Lago, MaríaJaitin, Diego AdhemarVillanueva, AlbertoVidal, AugustMartínez Martí, AlexFelip, EnriquetaVivancos Prellezo, AnaKeren-Shaul, HadasHeath, SimonGut, MartaAmit, IdoGut, Ivo GlynneHeyn, Holger2018-05-312018-05-312017Guillaumet-Adkins A, Rodríguez-Esteban G, Mereu E, Mendez-Lago M, Jaitin DA, Villanueva A et al. Single-cell transcriptome conservation in cryopreserved cells and tissues. Genome Biol. 2017 Mar 1;18(1):45. DOI: 10.1186/s13059-017-1171-91474-7596http://hdl.handle.net/10230/34756A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.application/pdfeng© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s13059-017-1171-9ConservationCryopreservationMars-seqPbmcPdoxPatient-derived orthotopic xenograftPeripheral blood mononuclear cellsRna sequencingSingle-cell genomicsSmart-seq2Transcriptomicsinfo:eu-repo/semantics/openAccess