García-Castro, HelenaKenny, Nathan J.Iglesias, MartaÁlvarez Campos, PatriciaMason, VincentElek, AnamariaSchönauer, AnnaSleight, Victoria A.Neiro, JakkeAboobaker, AzizPermanyer, JonIrimia Martínez, ManuelSebé-Pedrós, ArnauSolana, Jordi2021-05-192021-05-192021García-Castro H, Kenny NJ, Iglesias M, Álvarez-Campos P, Mason V, Elek A et al. ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics. Genome Biol. 2021 Apr 8;22(1):89. DOI: 10.1186/s13059-021-02302-51474-7596http://hdl.handle.net/10230/47612Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life.application/pdfeng© Helena García-Castro et al 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were madeGenèticaACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomicsinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s13059-021-02302-5info:eu-repo/semantics/openAccess