Kuhlwilm, MartinFontseré Alemany, Clàudia, 1992-Han, SojungAlvarez-Estape, MarinaMarquès i Bonet, Tomàs, 1975-2021-10-142021-10-142021Kuhlwilm M, Fontsere C, Han S, Alvarez-Estape M, Marques-Bonet T. HuConTest: testing human contamination in great ape samples. Genome Biol Evol. 2021;13(6):evab117. DOI: 10.1093/gbe/evab1171759-6653http://hdl.handle.net/10230/48646Modern human contamination is a common problem in ancient DNA studies. We provide evidence that this issue is also present in studies in great apes, which are our closest living relatives, for example in noninvasive samples. Here, we present a simple method to detect human contamination in short-read sequencing data from different species: HuConTest. We demonstrate its feasibility using blood and tissue samples from these species. This test is particularly useful for more complex samples (such as museum and noninvasive samples) which have smaller amounts of endogenous DNA, as we show here.application/pdfeng© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.HuConTest: testing human contamination in great ape samplesinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1093/gbe/evab117Ancient DNAContaminationFecal DNANext-generation sequencingNonhuman primatesinfo:eu-repo/semantics/openAccess