Baeza Delgado, Carlosvon Heijne, GunnarMartí Renom, Marc A.Mingarro, Ismael2016-06-022016-06-022016Baeza-Delgado C, von Heijne G, Marti-Renom MA, Mingarro I. Biological insertion of computationally designed short transmembrane segments. Scientific Reports. 2016; 6: 23397. DOI 10.1038/srep233972045-2322http://hdl.handle.net/10230/26804The great majority of helical membrane proteins are inserted co-translationally into the ER membrane through a continuous ribosome-translocon channel. The efficiency of membrane insertion depends on transmembrane (TM) helix amino acid composition, the helix length and the position of the amino acids within the helix. In this work, we conducted a computational analysis of the composition and location of amino acids in transmembrane helices found in membrane proteins of known structure to obtain an extensive set of designed polypeptide segments with naturally occurring amino acid distributions. Then, using an in vitro translation system in the presence of biological membranes, we experimentally validated our predictions by analyzing its membrane integration capacity. Coupled with known strategies to control membrane protein topology, these findings may pave the way to de novo membrane protein design.application/pdfeng© Nature Publishing Group. http://www.nature.com/articles/srep23397/nThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.Proteïnes de membranainfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/srep23397info:eu-repo/semantics/openAccess