Vernet-Tomás, MariaMena, MarisaAlemany, LaiaBravo, Ignacio G.Sanjosé Llongueras, Silvia deNicolau, PauBergueiro Flor, AlbaCorominas Torres, Josep MariaSerrano Figueras, SergiCarreras Collado, RamónLloveras Rubio, Belen2015-06-162015-06-162015Vernet-Tomas M, Mena M2, Alemany L3, Bravo I, De Sanjosé S, Nicolau P. et al. Human papillomavirus and breast cancer: no evidence of association in a Spanish set of cases. Anticancer Res. 2015 Feb;35(2):851-6.0250-7005http://hdl.handle.net/10230/23836BACKGROUND/AIM: Great controversy exists about the association between Human Papillomavirus (HPV) and breast tumors. The aim of this study was to explore the presence of HPV DNA in a large set of breast cancer cases. MATERIALS AND METHODS:/nTechniques used followed the standards for an international retrospective survey of HPV-DNA genotyping, coordinated by our own group and the DDL Laboratories in Rijswijk, the Netherlands. Paraffin-embedded samples were used. SPF-10 broad-spectrum primers were applied, followed by deoxyribonucleic acid enzyme immunoassay and genotyping by reverse-line probe assay./nRESULTS: A total of 78 samples were included in the study, 2 of benign conditions and 76 carcinomas, including different histological subtypes. HPV was not present in any of the specimens studied irrespective of histology, hormonal status and stage of disease. CONCLUSION: Our data do not support the involvement of HPV in breast carcinogenesis as no evidence of its presence was found.application/pdfengc) 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All reighs reserved.info:eu-repo/semantics/articleBreast cancerHuman papillomavirusPolymerase chain reactioninfo:eu-repo/semantics/openAccess