Mortuza, GulnaharCavazza, Tommaso, 1985-García Mayoral, María FlorHermida, DarioPeset, IsabelPedrero, Juan G.Merino, NekaneBlanco, FranciscoLyngsø, JeppeBruix, MartaPedersen, Jan SkovVernos, Isabelle, 1959-Montoya, Guillermo2020-12-012020-12-012014Mortuza GB, Cavazza T, Garcia-Mayoral MF, Hermida D, Peset I, Pedrero JG, Merino N, Blanco FJ, Lyngsø J, Bruix M, Pedersen JS, Vernos I, Montoya G. XTACC3-XMAP215 association reveals an asymmetric interaction promoting microtubule elongation. Nat Commun. 2014; 5:5072. DOI: 10.1038/ncomms6072041-1723http://hdl.handle.net/10230/45915chTOG is a conserved microtubule polymerase that catalyses the addition of tubulin dimers to promote microtubule growth. chTOG interacts with TACC3, a member of the transforming acidic coiled-coil (TACC) family. Here we analyse their association using the Xenopus homologues, XTACC3 (TACC3) and XMAP215 (chTOG), dissecting the mechanism by which their interaction promotes microtubule elongation during spindle assembly. Using SAXS, we show that the TACC domain (TD) is an elongated structure that mediates the interaction with the C terminus of XMAP215. Our data suggest that one TD and two XMAP215 molecules associate to form a four-helix coiled-coil complex. A hybrid methods approach was used to define the precise regions of the TACC heptad repeat and the XMAP215 C terminus required for assembly and functioning of the complex. We show that XTACC3 can induce the recruitment of larger amounts of XMAP215 by increasing its local concentration, thereby promoting efficient microtubule elongation during mitosis.application/pdfengThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/ncomms607MicrotubulesProteinsStructural biologyinfo:eu-repo/semantics/openAccess