Aranda Aragón, SergioBorràs, EvaSabidó Aguadé, Eduard, 1981-Di Croce, Luciano2020-11-182020-11-182020Aranda S, Borràs E, Sabidó E, Di Croce L. Chromatin-bound proteome profiling by genome capture. STAR Protoc. 2020; 1(1):100014. DOI: 10.1016/j.xpro.2020.1000142666-1667http://hdl.handle.net/10230/45793De novo identification of chromatin interactors can reveal unexpected pathways relevant to physiology and human disease. Inspired by the DNA mediated chromatin pull-down (Dm-ChP) technology (also known as iPOND [isolation of proteins on nascent DNA]) for the proteomic characterization of nascent DNA, we have recently reported a new experimental protocol that allows for the identification of proteins on total DNA (iPOTD) for bulk chromatome profiling and de novo identification of chromatin-bound proteins. Here, we detail a step-by-step protocol to survey the cellular chromatin-bound proteome in a simple, robust, and unbiased manner. For complete details on the use and execution of this protocol, please refer to Aranda et al. (2019).application/pdfeng© 2020. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.xpro.2020.100014info:eu-repo/semantics/openAccess