Castellví Fernández, QuimIvorra Cano, Antoni, 1974-2025-10-292025-10-292025Castellvi Q, Ivorra A. Computational multiscale modeling of pulsed field ablation considering conductivity and damage anisotropy reveals deep lesion morphologies. Int J Numer Method Biomed Eng. 2025 Aug;41(8):e70077. DOI: 10.1002/cnm.700772040-7939http://hdl.handle.net/10230/71689Pulsed Field Ablation (PFA) is an electroporation-based treatment modality to perform cardiac tissue ablations. Heart parenchyma is mainly constituted by elongated myocytes organized in fibers. This anisotropic morphology results in a preferential pathway for the electric current to flow along. Assuming conventional PFA modeling approaches in which lesions form where the electric field surpasses a threshold, such conductance anisotropy would result in relatively wide and shallow lesion morphologies when PFA applications are delivered with a focal monopolar catheter. Contrary to that, some recent preclinical data present narrow and deep elongated lesions. This study presents a multiscale simulation approach able to estimate electroporation treatment outcomes when applied in a highly anisotropic tissue such as the myocardium. In this work, a microscopic model was first implemented mimicking the conformation of the cardiac tissue. Longitudinal and transversal electric fields at different frequencies and magnitudes were applied to characterize the expected anisotropic behavior at the tissue level in terms of electric conductivity and expected membrane disruption due to electroporation. Second, the microscopic characterization was integrated into a macroscopic model of a focal ablation catheter in contact with the myocardial tissue to simulate the delivery of monopolar PFA treatments. The microscopic simulations results show that when low electric field magnitudes are applied, the induced membrane disruptions predominantly appear in fibers parallel to the electric field. However, at higher field magnitudes, a demarcated superior sensitivity is observed in perpendicular orientation. The integration of these anisotropic properties into the macroscopic model predicts width/depth ratios of 1.2 compared to the ratios of about 2 predicted with conventional modeling. In this work, the presented multiscale model and approach can predict relatively narrow and deep lesions, as observed preclinically.application/pdfeng© 2025 The Author(s). International Journal for Numerical Methods in Biomedical Engineering published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in anymedium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.info:eu-repo/semantics/article2025-10-29http://dx.doi.org/10.1002/cnm.70077AnisotropyCardiac ablationMathematical modelMultiscale modelingNumerical modelPFAPulsed field ablationinfo:eu-repo/semantics/openAccess