Chen, LuBreschi, Alessandra, 1988-Guigó Serra, RodericPalumbo, EmilioRendon, Augusto2019-06-202019-06-202014Chen L, Kostadima M, Martens JHA, Canu G, Garcia SP, Turro E et al. Transcriptional diversity during lineage commitment of human blood progenitors. Science. 2014 Sep 26;345(6204):1251033. DOI: 10.1126/science.12510331095-9203http://hdl.handle.net/10230/41853Blood cells derive from hematopoietic stem cells through stepwise fating events. To characterize gene expression programs driving lineage choice, we sequenced RNA from eight primary human hematopoietic progenitor populations representing the major myeloid commitment stages and the main lymphoid stage. We identified extensive cell type-specific expression changes: 6711 genes and 10,724 transcripts, enriched in non-protein-coding elements at early stages of differentiation. In addition, we found 7881 novel splice junctions and 2301 differentially used alternative splicing events, enriched in genes involved in regulatory processes. We demonstrated experimentally cell-specific isoform usage, identifying nuclear factor I/B (NFIB) as a regulator of megakaryocyte maturation-the platelet precursor. Our data highlight the complexity of fating events in closely related progenitor populations, the understanding of which is essential for the advancement of transplantation and regenerative medicine.application/pdfengThis is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science on vol. 345, num. 6204, 2014. DOI: 10.1126/science.1251033Empalmament alternatiullinatge cel·lularHematopoesiCèl·lules mare hematopoètiquesinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1126/science.1251033info:eu-repo/semantics/openAccess