Thomassin, Jenny-LeeSantos Moreno, JavierGuilvout, IngridTran Van Nhieu, GuyFrancetic, Olivera2025-07-162025-07-162017Thomassin JL, Santos Moreno J, Guilvout I, Tran Van Nhieu G, Francetic O. The trans-envelope architecture and function of the type 2 secretion system: new insights raising new questions. Mol Microbiol. 2017 Jul;105(2):211-26. DOI: 10.1111/mmi.137040950-382Xhttp://hdl.handle.net/10230/70934Nanomachines belonging to the type IV filament (Tff) superfamily serve a variety of cellular functions in prokaryotes, including motility, adhesion, electrical conductance, competence and secretion. The type 2 secretion system (T2SS) Tff member assembles a short filament called pseudopilus that promotes the secretion of folded proteins from the periplasm across the outer membrane of Gram-negative bacteria. A combination of structural, biochemical, imaging, computational and in vivo approaches had led to a working model for the assembled nanomachine. High-resolution cryo-electron microscopy and tomography provided the first view of several homologous Tff nanomachines in the cell envelope and revealed the structure of the outer membrane secretin channel, challenging current models of the overall stoichiometry of the T2SS. In addition, recent insights into exoprotein substrate features and interactions with the T2SS have led to new questions about the dynamics of the system and the role of the plasma membrane in substrate presentation. This micro-review will highlight recent advances in the field of type 2 secretion and discuss approaches that can be used to reach a mechanistic understanding of exoprotein recognition, integration into the machine and secretion.application/pdfengThis is the peer reviewed version of the following article: Thomassin JL, Santos Moreno J, Guilvout I, Tran Van Nhieu G, Francetic O. The trans-envelope architecture and function of the type 2 secretion system: new insights raising new questions. Mol Microbiol. 2017 Jul;105(2):211-26. DOI: 10.1111/mmi.13704, which has been published in final form at http://dx.doi.org/10.1111/mmi.13704. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.Secrecióinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/mmi.13704info:eu-repo/semantics/openAccess