Xia, YingchenZha, JianhuaCurull Serrano, VíctorSánchez-Font, AlbertGuitart, MariaRodríguez-Fuster, AlbertoAguiló Espases, RafaelBarreiro Portela, Esther2023-02-032023-02-032022Xia Y, Zha J, Curull V, Sánchez-Font A, Guitart M, Rodríguez-Fuster A, et al. Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPD. ERJ Open Res. 2022 Jul 18; 8(3): 00105-2022. DOI: 10.1183/23120541.00105-20222312-0541http://hdl.handle.net/10230/55604Epithelial-mesenchymal transition (EMT) is involved in the pathophysiology of lung cancer (LC) and COPD, and the latter is an important risk factor for LC. We hypothesised that the EMT gene expression profile and signalling cascade may differ in LC patients with COPD from those with no respiratory diseases. In lung tumour specimens obtained through video-assisted thoracoscopic surgery from LC (n=20, control group) and LC-COPD patients (n=30), gene expression (quantitative real-time PCR amplification) of EMT markers SMAD3, SMAD4, ZEB2, TWIST1, SNAI1, ICAM1, VIM, CDH2, MMP1 and MMP9 was detected. In lung tumours of LC-COPD compared to LC patients, gene expression of SMAD3, SMAD4, ZEB2 and CDH2 significantly declined, while no significant differences were detected for the other analysed markers. A significant correlation was found between pack-years (smoking burden) and SMAD3 gene expression among LC-COPD patients. LC-COPD patients exhibited mild-to-moderate airway obstruction and a significant reduction in diffusion capacity compared to LC patients. In lung tumour samples of patients with COPD, several markers of EMT expression, namely SMAD3, SMAD4, ZEB2 and CDH2, were differentially expressed suggesting that these markers are likely to play a role in the regulation of EMT in patients with this respiratory disease. Cigarette smoke did not seem to influence the expression of EMT markers in this study. These results have potential clinical implications in the management of patients with LC, particularly in those with underlying respiratory diseases.application/pdfengThis version is distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. (http://creativecommons.org/licenses/by/4.0/). For commercial reproduction rights and permissions contact permissions@ersnet.orgPulmons--CàncerGenèticaGensAparell respiratori--Malaltiesinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1183/23120541.00105-2022info:eu-repo/semantics/openAccess