García Santamarina, Sarela, 1978-Boronat i Llop, Susanna, 1965-Hidalgo Hernando, Elena2016-01-132016-01-132014García-Santamarina S, Boronat S, Hidalgo E. Reversible cysteine oxidation in hydrogen peroxide sensing and signal transduction. Biochemistry. 2014;53(16):2560-80. DOI: 10.1021/bi401700f0006-2960http://hdl.handle.net/10230/25566Activation of redox cascades through hydrogen peroxide-mediated reversible cysteine oxidation is a major mechanism for intracellular signaling. Understanding why some cysteine residues are specifically oxidized, in competition with other proximal cysteine residues and in the presence of strong redox buffers, is therefore crucial for understanding redox signaling. In this review, we explore the recent advances in thiol-redox chemistry linked to signaling. We describe the last findings in the field of redox sensors, those that are naturally present in different model organisms as well as those that have been engineered to quantify intracellular hydrogen peroxide concentrations. Finally, we provide a summary of the newest approaches developed to study reversible cysteine oxidation at the proteomic level.application/pdfeng© American Chemical Society after peer review and technical editing by the publisher./nTo access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/bi401700fOxigen actiuProteòmicainfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1021/bi401700fRedox biologyH2O2 reactivityCys oxidationH2O2 sensorRedox proteomicsPap1Yap1OxyRNrf2roGFPinfo:eu-repo/semantics/openAccess