Chiva, CristinaSabidó Aguadé, Eduard, 1981-2018-06-142018-06-142017Chiva C, Sabidó E. Peptide Selection for Targeted Protein Quantitation. J Proteome Res. 2017 Mar 3;16(3):1376-80. DOI: 10.1021/acs.jproteome.6b00115. Epub 2017 Jan 301535-3893http://hdl.handle.net/10230/34897Targeted proteomics methods in their different flavors rely on the use of a few peptides as proxies for protein quantitation, which need to be specified either prior to or after data acquisition. However, in contrast with discovery methods that use all identified peptides for a given protein to estimate its abundance, targeted proteomics methods are limited in the number of peptides that are used for protein quantitation. Because only a few peptides per protein are acquired or extracted in targeted experiments, the selection of peptides that are used for targeted protein quantitation becomes crucial. Several rules have been proposed to guide peptide selection for targeted proteomics studies, which have generally been based on the amino acidic composition of the peptide sequences. However, the compliance of these rules does not imply that not-conformed peptides are not reproducibly generated nor do they guarantee that the selected peptides correctly represent the behavior of the protein abundance under different conditions.application/pdfengThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Proteome Res., copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jproteome.6b00115info:eu-repo/semantics/articlehttp://dx.doi.org/10.1021/acs.jproteome.6b00115Data independent acquisitionSelected reaction monitoringTargeted proteomicsQuantitationinfo:eu-repo/semantics/openAccess