Gaviria, Loreno PatríciaMontsant, LourdesAzuaje, CarlosGonzález-Díaz, AidaHorcajada Gallego, Juan PabloLimón, EnricViñas, MiguelEspinal, PaulaFusté, Ester2022-10-102022-10-102022Gaviria LP, Montsant L, Azuaje C, González-Díaz A, Horcajada JP, Limón E, Viñas M, Espinal P, Fusté E. A descriptive analysis of urinary ESBL-producing- Escherichia coli in Cerdanya Hospital. Microorganisms. 2022 Feb 22;10(3):488. DOI: 10.3390/microorganisms100304882076-2607http://hdl.handle.net/10230/54319Urinary tract infections caused by extended-spectrum β-lactamase Escherichia coli (ESBL-EC) are increasing worldwide and are a current concern because treatment options are often limited. This study investigated antimicrobial susceptibility, antimicrobial resistance genes (ARGs), and the biological diversity of urinary ESBL-EC isolates at Cerdanya Hospital, a European cross-border hospital that combines French and Spanish healthcare models. Bacterial identification and susceptibility were determined using the Microscan WalkAway® system and ESBL production was examined by the double-disk synergy method. Isolates were sequenced using the Ion S5™ next-generation sequencing system, with the whole-genome sequences then assembled using SPADEs software and analyzed using PubMLST, ResFinder, FimTyper, PlasmidFinder, and VirulenceFinder. A phylogenetic analysis was performed by constructing an assembly-based core-SNV alignment, followed by a phylogenetic tree constructed using Parsnp from the Harvest suite. All isolates studied were multidrug-resistant and could be classified into 19 different sequence types characterized by a high genetic diversity. The most prevalent ESBL-enzymes were CTX-M-14 and CTX-M-15. High-risk international clones (ST131, ST10, and ST405) were also identified. The results demonstrated the absence of a single predominant clone of ESBL-MDR-EC at Cerdanya Hospital.application/pdfeng© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/microorganisms10030488Escherichia coliExtended-spectrum β-lactamase (ESBL)Molecular characterizationMultidrug resistance (MDR)Sequence type (ST)Urinary tract infections (UTI)Whole-genome sequencing (WGS)info:eu-repo/semantics/openAccess