Martínez Cruz, BegoñaZiegle, JanetSanz, PaulaSotelo, GracielaAnglada Busquets, RogerPlaza, StéphanieComas, David, 1969-2014-12-222014-12-222011Martínez-Cruz B, Ziegle J, Sanz P, Sotelo G, Anglada R, Plaza S, Comas D. Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes. Investigative Genetics. 2011 May;2:13. DOI: 10.1186/2041-2223-2-132041-2223http://hdl.handle.net/10230/23033Background: The analysis of human Y-chromosome variation in the context of population genetics and forensics requires the genotyping of dozens to hundreds of selected single-nucleotide polymorphisms (SNPs). In the present study, we developed a 121-plex (121 SNPs in a single array) TaqMan array capable of distinguishing most haplogroups and subhaplogroups on the Y-chromosome human phylogeny in Europe./nResults: We present data from 264 samples from several European areas and ethnic groups. The array developed in this study shows >99% accuracy of assignation to the Y human phylogeny (with an average call rate of genotypes >96%)./nConclusions: We have created and evaluated a robust and accurate Y-chromosome multiplex which minimises the possible errors due to mixup when typing the same sample in several independent reactions.application/pdfeng© 2011 Martínez-Cruz et al; licensee BioMed Central Ltd. /nThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Cromosoma XCromosoma YGenòmicaFilogèniainfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/2041-2223-2-13info:eu-repo/semantics/openAccess