Zaffagnini, GabrieleCheng, ShiyaSalzer, Marion C.Pernaute, BarbaraDuran, Juan M.Irimia Martínez, ManuelSchuh, MelinaBöke, Elvan2024-07-232024-07-232024Zaffagnini G, Cheng S, Salzer MC, Pernaute B, Duran JM, Irimia M, et al. Mouse oocytes sequester aggregated proteins in degradative super-organelles. Cell. 2024 Feb 29;187(5):1109-26.e21. DOI: 10.1016/j.cell.2024.01.0310092-8674http://hdl.handle.net/10230/60808Oocytes are among the longest-lived cells in the body and need to preserve their cytoplasm to support proper embryonic development. Protein aggregation is a major threat for intracellular homeostasis in long-lived cells. How oocytes cope with protein aggregation during their extended life is unknown. Here, we find that mouse oocytes accumulate protein aggregates in specialized compartments that we named endolysosomal vesicular assemblies (ELVAs). Combining live-cell imaging, electron microscopy, and proteomics, we found that ELVAs are non-membrane-bound compartments composed of endolysosomes, autophagosomes, and proteasomes held together by a protein matrix formed by RUFY1. Functional assays revealed that in immature oocytes, ELVAs sequester aggregated proteins, including TDP-43, and degrade them upon oocyte maturation. Inhibiting degradative activity in ELVAs leads to the accumulation of protein aggregates in the embryo and is detrimental for embryo survival. Thus, ELVAs represent a strategy to safeguard protein homeostasis in long-lived cells.application/pdfeng© 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.cell.2024.01.031RUFY1EmbryoFemale fertilityLysosomal acidificationMembraneless organellesOocyteOocyte qualityProtein aggregationProteostasisSuper-organellesinfo:eu-repo/semantics/openAccess