Galão, Rui Pedro RibeiroChari, AshwinAlves-Rodrigues, IsabelLobão, DanielaMas, Antonio JuanKambach, ChristianFischer, UtzDíez Antón, Juana, 1962-2016-01-192016-01-192010Galão RP, Chari A, Alves-Rodrigues I, Lobão D, Mas A, Kambach C et al. LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication. RNA. 2010;16(4):817-27. DOI: 10.1261/rna.17129101355-8382http://hdl.handle.net/10230/25602LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3'-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.application/pdfengCopyright © 2010, RNA by RNA Society. Published version available at http://rnajournal.cshlp.org/content/16/4/817.longInteraccions RNA-proteïnaSaccharomyces cerevisiae -- Metabolismeinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1261/rna.1712910BMVLSm1-7RNA virusSm proteinsTranslationReplicationinfo:eu-repo/semantics/openAccess