Martin-Cofreces, NoaChichon, Francisco JavierCalvo, EnriqueTorralba, DanielBustos Moran, EugenioDosil, Sara G.Rojas-Gomez, AmeliaBonzón-Kulichenko, ElenaLopez, J. A.Oton, JoaquinSorrentino, AndreaZabala, Juan C.Vernos, Isabelle, 1959-Vazquez, J.Valpuesta, José M.Sanchez-Madrid, Francisco2022-06-132022-06-132020Martin-Cofreces NB, Chichon FJ, Calvo E, Torralba D, Bustos-Moran E, Dosil SG et al. The chaperonin CCT controls T cell receptor-driven 3D configuration of centrioles. Sci Adv. 2020 Dec 2;6(49):eabb7242. DOI:10.1126/sciadv.abb72422375-2548http://hdl.handle.net/10230/53472T lymphocyte activation requires the formation of immune synapses (IS) with antigen-presenting cells. The dynamics of membrane receptors, signaling scaffolds, microfilaments, and microtubules at the IS determine the potency of T cell activation and subsequent immune response. Here, we show that the cytosolic chaperonin CCT (chaperonin-containing TCP1) controls the changes in reciprocal orientation of the centrioles and polarization of the tubulin dynamics induced by T cell receptor in T lymphocytes forming an IS. CCT also controls the mitochondrial ultrastructure and the metabolic status of T cells, regulating the de novo synthesis of tubulin as well as posttranslational modifications (poly-glutamylation, acetylation, Δ1 and Δ2) of αβ-tubulin heterodimers, fine-tuning tubulin dynamics. These changes ultimately determine the function and organization of the centrioles, as shown by three-dimensional reconstruction of resting and stimulated primary T cells using cryo-soft x-ray tomography. Through this mechanism, CCT governs T cell activation and polarity.application/pdfeng© 2020 N. B. Martin-Cofreces et al, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC)GenèticaCèl·lules TSistema immunològicXaperoninesinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1126/sciadv.abb7242info:eu-repo/semantics/openAccess