Roman-Canal, BertaTarragona, JordiMoiola, Cristian PabloGatius, SòniaBonnin, SarahRuiz-Miró, MariaSierra, José EnriqueRufas, MariaGonzález, EsperanzaPorcel, José M.Gil-Moreno, AntonioFalcón-Pérez, Juan M.Ponomarenko, JuliaMatias-Guiu, XavierColas, Eva2019-09-202019-09-202019Roman-Canal B, Tarragona J, Moiola CP, Gatius S, Bonnin S, Ruiz-Miró M et al. EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer. J Transl Med. 2019;17(1):208. DOI: 10.1186/s12967-019-1954-81479-5876http://hdl.handle.net/10230/42305Background: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current systematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identification of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. Methods: In this study, we aim to develop a novel approach to identify highly sensitive and specific biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fluids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. Results: 210 miRNAs were significantly dysregulated (adjusted p value < 0.05 and abs(logFC) ≥ 1). The top-10 miRNAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. Conclusions: This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.application/pdfeng© 2019, Berta Roman-Canal et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s12967-019-1954-8Colorectal cancerBiomarkersDiagnosticmiRNAsAscitic fluidPeritoneal lavageLiquid biopsyExtracellular vesiclesColon cancerinfo:eu-repo/semantics/openAccess