Miralles-Pérez, BernatRamos-Romero, SaraCharpentier, María JoséSánchez-Martos, VanessaFortuño-Mar, ÀngelsPonomarenko, JuliaAmézqueta, SusanaPiñol-Piñol, DavidZhang, XiangTorres, Josep LluísRomeu, Marta2025-10-142025-10-142025Miralles-Pérez B, Ramos-Romero S, Charpentier MJ, Sánchez-Martos V, Fortuño-Mar À, Ponomarenko J, et al. Dietary consumption of Type 2 resistant starch and d-fagomine delays progression of metabolic disturbances in male rats on high-fat diet. Mol Nutr Food Res. 2025 Sep 9:e70230. DOI: 10.1002/mnfr.702301613-4125http://hdl.handle.net/10230/71494Data de publicació electrònica: 09-09-2025High-fat (HF) diets contribute to obesity, insulin resistance, fatty liver, gut microbiota dysbiosis, oxidative stress, and low-grade chronic inflammation. This study evaluated the preventive effects of dietary Type 2 resistant starch (RS2) from high-amylose maize and low-dose d-fagomine (FG) from buckwheat on these metabolic disturbances. Male Wistar-Kyoto rats (9-10 weeks old) were assigned to four diet groups for 10 weeks: standard (STD) diet, HF diet (45% kcal from fat), HF + RS diet (15% RS2), and HF + FG diet (0.1% FG). Body characteristics, metabolic parameters, oxidative stress, gut microbiota, short-chain fatty acids (SCFAs), and eicosanoids were analyzed. Both HF + RS and HF + FG diets reduced perigonadal fat, plasma triacylglycerols, and oxidative stress. HF + RS diet improved glucose tolerance without significantly affecting insulin sensitivity, while HF + FG diet showed a tendency for improvement at later stages. Additionally, HF + RS diet showed greater beneficial effects on body weight and liver steatosis than HF + FG diet, likely due to gut microbiota and SCFA modulation. RS2 exerted stronger metabolic effects than FG under HF diet conditions, suggesting its greater potential in mitigating obesity-related complications. FG effects may require longer exposure to manifest.application/pdfeng© 2025 The Author(s). Molecular Nutrition & Food Research published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/mnfr.70230Atherogenic dyslipidemiaFiberIminosugarOxidative stressPrediabetesinfo:eu-repo/semantics/openAccess