Otto, ChristianeKovalchuk, YuryWolfer, David PaulGass, PeterMartín, MiguelZuschratter, WernerGröne, Hermann JosefKellendonk, ChristophTronche, FrançoisMaldonado, Rafael, 1961-Lipp, Hans-PeterKonnerth, ArthurSchütz, Günter2012-07-052012-07-052001Otto C, Kovalchuk Y, Wolfer DP, Gass P, Martin M, Zuschratter W et al. Impairment of mossy fiber long-term potentiation and associative learning in pituitary adenylate cyclase activating polypeptide type I receptor-deficient Mice. J Neurosci. 2001;21(15):5520-7. DOI: 10.1523/jneurosci.21-15-05520.20010270-6474http://hdl.handle.net/10230/16668The pituitary adenylate cyclase activating polypeptide (PACAP) type I receptor (PAC1) is a G-protein-coupled receptor binding the strongly conserved neuropeptide PACAP with 1000-fold higher affinity than the related peptide vasoactive intestinal peptide. PAC1-mediated signaling has been implicated in neuronal differentiation and synaptic plasticity. To gain further insight into the biological significance of PAC1-mediated signaling in vivo, we generated two different mutant mouse strains, harboring either a complete or a forebrain-specific inactivation of PAC1. Mutants from both strains show a deficit in contextual fear conditioning, a hippocampus-dependent associative learning paradigm. In sharp contrast, amygdala-dependent cued fear conditioning remains intact. Interestingly, no deficits in other hippocampus-dependent tasks modeling declarative learning such as the Morris water maze or the social transmission of food preference are observed. At the cellular level, the deficit in hippocampus-dependent associative learning is accompanied by an impairment of mossy fiber long-term potentiation (LTP). Because the hippocampal expression of PAC1 is restricted to mossy fiber terminals, we conclude that presynaptic PAC1-mediated signaling at the mossy fiber synapse is involved in both LTP and hippocampus-dependent associative learning.application/pdfeng© 2001, Society for Neuroscience. The published version is available at: http://www.jneurosci.org/content/21/15/5520Xarxes neuronals (Neurobiologia)Neuropèptidsinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1523/jneurosci.21-15-05520.2001PACAP type I receptorKnock-out miceFear conditioningSynaptic plasticityLTPMossy fiberinfo:eu-repo/semantics/openAccess