Chafino Aixa, Silvia, 1991-Ureña, EnricCasanova, JordiCasacuberta Suñer, Elena, 1972-Franch i Marro, XavierMartín, David2020-05-262020-05-262019Chafino S, Ureña E, Casanova J, Casacuberta E, Franch-Marro X, Martín D. Upregulation of E93 gene expression acts as the trigger for metamorphosis independently of the threshold size in the beetle tribolium castaneum. Cell Rep. 2019; 27(4):1039-1049.e2. DOI: 10.1016/j.celrep.2019.03.0942211-1247http://hdl.handle.net/10230/44808Body size in holometabolous insects is determined by the size at which the juvenile larva undergoes metamorphosis to the pupal stage. To undergo larva-pupa transition, larva must reach a critical developmental checkpoint, the threshold size (TS); however, the molecular mechanisms through which the TS cues this transition remain to be fully characterized. Here, we use the flour beetle Tribolium castaneum to characterize the molecular mechanisms underlying entry into metamorphosis. We found that T. castaneum reaches a TS at the beginning of the last larval instar, which is associated with the downregulation of TcKr-h1 and the upregulation of TcE93 and TcBr-C. Unexpectedly, we found that while there is an association between TS and TcE93 upregulation, it is the latter that constitutes the molecular trigger for metamorphosis initiation. In light of our results, we evaluate the interactions that control the larva-pupa transition and suggest alternative models.application/pdfeng© 2019 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.celrep.2019.03.094E93Kr-h1Tribolium castaneumBroad-complexEcdysoneGrowth controlHolometabolyInsect metamorphosisJuvenile hormoneThreshold sizeinfo:eu-repo/semantics/openAccess