Mighell, Taylor L.Toledano, IgnasiLehner, Ben, 1978-2023-11-032023-11-032023Mighell TL, Toledano I, Lehner B. SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries. PLoS One. 2023 Jul 7;18(7):e0288158. DOI: 10.1371/journal.pone.02881581932-6203http://hdl.handle.net/10230/58204Multiplexed assays of variant effects (MAVEs) have made possible the functional assessment of all possible mutations to genes and regulatory sequences. A core pillar of the approach is generation of variant libraries, but current methods are either difficult to scale or not uniform enough to enable MAVEs at the scale of gene families or beyond. We present an improved method called Scalable and Uniform Nicking (SUNi) mutagenesis that combines massive scalability with high uniformity to enable cost-effective MAVEs of gene families and eventually genomes.application/pdfeng© 2023 Mighell et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0288158MutagenesisLibrary screeningPolymerase chain reactionDNA synthesisNucleotidesOligonucleotidesMutationPlasmid constructioninfo:eu-repo/semantics/openAccess