Lerner, JonathanGómez García, PabloMcCarthy, Ryan L.Liu, ZheLakadamyali, MelikeZaret, Kenneth S.2022-06-142022-06-142020Lerner J, Gómez-García PA, McCarthy RL, Liu Z, Lakadamyali M, Zaret KS. Two-parameter single-molecule analysis for measurement of chromatin mobility. STAR Protoc. 2020 Dec 13;1(3):100223. DOI:10.1016/j.xpro.2020.1002232666-1667http://hdl.handle.net/10230/53489This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020).application/pdfeng© Jonathan Lerner et al 2020. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)CromatinaProteïnesMolèculesinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.xpro.2020.100223info:eu-repo/semantics/openAccess