Vilahur Chiaraviglio, Nadia, 1982-Bustamante Pineda, MarionaMorales, EvaMotta, ValeriaFernandez, Mariana F.Salas, Lucas A.Escaramís, GeòrgiaBallester Díez, FerranMurcia, MarioTardón, AdoninaRiaño, IsolinaSanta Marina, LoretoIbarluzea, JesúsArrebola, Juan PedroEstivill, Xavier, 1955-Bollati, ValentinaSunyer Deu, JordiOlea, Nicolás2016-05-232017-02-012016Vilahur N, Bustamante M, Morales E, Motta V, Fernandez MF, Salas LA et al. Prenatal exposure to mixtures of xenoestrogens and genome-wide DNA methylation in human placenta. Epigenomics. 2016; 8(1): 43-54. DOI 10.2217/epi.15.911750-1911http://hdl.handle.net/10230/26427BACKGROUND: In utero exposure to xenostrogens may modify the epigenome. We explored the association of prenatal exposure to mixtures of xenoestrogens and genome-wide placental DNA methylation. MATERIALS & METHODS: Sex-specific associations between methylation changes in placental DNA by doubling the concentration of TEXB-alpha exposure were evaluated by robust multiple linear regression. Two CpG sites were selected for validation and replication in additional male born placentas. RESULTS: No significant associations were found, although the top significant CpGs in boys were located in the LRPAP1, HAGH, PPARGC1B, KCNQ1 and KCNQ1DN genes, previously associated to birth weight, Type 2 diabetes, obesity or steroid hormone signaling. Neither technical validation nor biological replication of the results was found in boys for LRPAP and PPARGC1B. CONCLUSION: Some suggestive genes were differentially methylated in boys in relation to prenatal xenoestrogen exposure, but our initial findings could not be validated or replicated.application/pdfeng© Future Medicine http://dx.doi.org/10.2217/epi.15.91ADNPlacentainfo:eu-repo/semantics/articlehttp://dx.doi.org/10.2217/epi.15.91DNA methylationTEXBEndocrine disruptorsEpigenome xenoestrogensPlacentaPrenatalProgramminginfo:eu-repo/semantics/openAccess