Arumugam, KarthikShin, WilliamSchiavone, ValentinaVlahos, LukasTu, XiaochuanCarnevali, DavideKesner, JordanPaull, Evan O.Romo Saladrigas, Neus, 1982-Subramanian, PremWorley, JeremyTan, XiangtianCalifano, AndreaCosma, Maria Pia, 1970-2021-03-172021-03-172020Arumugam K, Shin W, Schiavone V, Vlahos L, Tu X, Carnevali D et al. The master regulator protein BAZ2B can reprogram human hematopoietic lineage-committed progenitors into a multipotent state. Cell Rep. 2020 Dec 8; 33(10): 108474. DOI: 10.1016/j.celrep.2020.1084742211-1247http://hdl.handle.net/10230/46819Bi-species, fusion-mediated, somatic cell reprogramming allows precise, organism-specific tracking of unknown lineage drivers. The fusion of Tcf7l1-/- murine embryonic stem cells with EBV-transformed human B cell lymphocytes, leads to the generation of bi-species heterokaryons. Human mRNA transcript profiling at multiple time points permits the tracking of the reprogramming of B cell nuclei to a multipotent state. Interrogation of a human B cell regulatory network with gene expression signatures identifies 8 candidate master regulator proteins. Of these 8 candidates, ectopic expression of BAZ2B, from the bromodomain family, efficiently reprograms hematopoietic committed progenitors into a multipotent state and significantly enhances their long-term clonogenicity, stemness, and engraftment in immunocompromised mice. Unbiased systems biology approaches let us identify the early driving events of human B cell reprogramming.application/pdfeng© 2020 Karthik Arumugam et al. Open access under a Creative Commons licenseGenèticaCèl·lules mareProteïnesinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.celrep.2020.108474info:eu-repo/semantics/openAccess