Ramos-Romero, SaraPonomarenko, JuliaAmézqueta, SusanaHereu, MercèMiralles-Pérez, BernatRomeu, MartaMéndez, LucíaMedina, IsabelTorres, Josep Lluís2023-01-242023-01-242022Ramos-Romero S, Ponomarenko J, Amézqueta S, Hereu M, Miralles-Pérez B, Romeu M, Méndez L, Medina I, Torres JL. Fiber-like action of d-fagomine on the gut microbiota and body weight of healthy rats. Nutrients. 2022 Nov 3;14(21):4656. DOI: 10.3390/nu142146562072-6643http://hdl.handle.net/10230/55414The goal of this work is to explore if the changes induced by d-fagomine in the gut microbiota are compatible with its effect on body weight and inflammation markers in rats. Methods: Sprague Dawley rats were fed a standard diet supplemented with d-fagomine (or not, for comparison) for 6 months. The variables measured were body weight, plasma mediators of inflammation (hydroxyeicosatetraenoic acids, leukotriene B4, and IL-6), and the concentration of acetic acid in feces and plasma. The composition and diversities of microbiota in cecal content and feces were estimated using 16S rRNA metabarcoding and high-throughput sequencing. We found that after just 6 weeks of intake d-fagomine significantly reduced body weight gain, increased the plasma acetate concentration, and reduced the plasma concentration of the pro-inflammatory biomarkers' leukotriene B4, interleukin 6 and 12 hydroxyeicosatetraenoic acids. These changes were associated with a significantly increased prevalence of Bacteroides and Prevotella feces and increased Bacteroides, Prevotella, Clostridium, and Dysgonomonas while reducing Anaerofilum, Blautia, and Oribacterium in cecal content. In conclusion, d-fagomine induced changes in the composition and diversity of gut microbiota similar to those elicited by dietary fiber and compatible with its anti-inflammatory and body-weight-reducing effects.application/pdfeng© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/nu14214656Body weightd-fagomineGut microbiotaIminocyclitolIminosugarInflammationinfo:eu-repo/semantics/openAccess