Di Stefano, MarcoStadhouders, RalphFarabella, IreneCastillo Andreo, DavidSerra, FrançoisGraf, T. (Thomas)Marti-Renom, Marc A.2020-10-222020-10-222020Marco S, Stadhouders R, Farabella I, Castillo D, SerraF, Graf T et al. Transcriptional activation during cell reprogramming correlates with the formation of 3D open chromatin hubs. Nat Commun. 2020 May 22; 11(1): 2564. DOI: 10.1038/s41467-020-16396-12041-1723http://hdl.handle.net/10230/45554Chromosome structure is a crucial regulatory factor for a wide range of nuclear processes. Chromosome conformation capture (3C)-based experiments combined with computational modelling are pivotal for unveiling 3D chromosome structure. Here, we introduce TADdyn, a tool that integrates time-course 3C data, restraint-based modelling, and molecular dynamics to simulate the structural rearrangements of genomic loci in a completely data-driven way. We apply TADdyn on in situ Hi-C time-course experiments studying the reprogramming of murine B cells to pluripotent cells, and characterize the structural rearrangements that take place upon changes in the transcriptional state of 21 genomic loci of diverse expression dynamics. By measuring various structural and dynamical properties, we find that during gene activation, the transcription starting site contacts with open and active regions in 3D chromatin domains. We propose that these 3D hubs of open and active chromatin may constitute a general feature to trigger and maintain gene transcription.application/pdfeng© Marco Di Stefano et al. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were madeCèl·lulesCromosomesinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41467-020-16396-1info:eu-repo/semantics/openAccess