Martin, LauraVicario, ChiaraCastells García, Àlvaro, 1991-Lakadamyali, MelikeNeguembor, Maria VictoriaCosma, Maria Pia2022-01-312022-01-312021Martin L, Vicario C, Castells-García A, Lakadamyali M, Neguembor MV, Cosma MP. A protocol to quantify chromatin compaction with confocal and super-resolution microscopy in cultured cells. STAR Protoc. 2021 Sep 30; 2(4): 100865. DOI: 10.1016/j.xpro.2021.1008652666-1667http://hdl.handle.net/10230/52374Here, we describe three complementary microscopy-based approaches to quantify morphological changes of chromatin organization in cultured adherent cells: the analysis of the coefficient of variation of DNA, the measurement of DNA-free nuclear areas, and the quantification of chromatin-associated proteins at the nuclear edge. These approaches rely on confocal imaging and stochastic optical reconstruction microscopy and allow a fast and robust quantification of chromatin compaction. These approaches circumvent inter-variability between imaging conditions and apply to every type of adherent cells. For complete details on the use and execution of this protocol, please refer to Neguembor et al. (2021).application/pdfeng© 2021 Laura Martin et al. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)CitologiaMicroscòpiaBiologia molecularinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.xpro.2021.100865info:eu-repo/semantics/openAccess