Chen, Wei-Huavan Noort, VeraLluch-Senar, Maria 1982-Hennrich, Marco L.Wodke, Judith A.H.Yus, EvaAlibés, AndreuRoma, GuglielmoMende, Daniel R.Pesavento, ChristinaTypas, AthanasiosGavin, Anne-ClaudeSerrano Pubull, Luis, 1982-2016-06-022016-06-022016Chen WH, van Noort V, Lluch-Senar M, Hennrich ML, H Wodke JA, Yus E et al. Integration of multi-omics data of a genome-reduced bacterium: Prevalence of post-transcriptional regulation and its correlation with protein abundances. Nucleic Acids Research. 2016; 44(3): 1192-1202. DOI 10.1093/nar/gkw0041759-6653http://hdl.handle.net/10230/26807We developed a comprehensive resource for the genome-reduced bacterium Mycoplasma pneumoniae comprising 1748 consistently generated '-omics' data sets, and used it to quantify the power of antisense non-coding RNAs (ncRNAs), lysine acetylation, and protein phosphorylation in predicting protein abundance (11%, 24% and 8%, respectively). These factors taken together are four times more predictive of the proteome abundance than of mRNA abundance. In bacteria, post-translational modifications (PTMs) and ncRNA transcription were both found to increase with decreasing genomic GC-content and genome size. Thus, the evolutionary forces constraining genome size and GC-content modify the relative contributions of the different regulatory layers to proteome homeostasis, and impact more genomic and genetic features than previously appreciated. Indeed, these scaling principles will enable us to develop more informed approaches when engineering minimal synthetic genomes.application/pdfeng© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research./nThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1093/nar/gkw004info:eu-repo/semantics/openAccess