García Rodríguez, PatriciaEncinar del Dedo, JavierAyté del Olmo, JoséHidalgo Hernando, Elena2016-01-132017-01-082016García P, Encinar Del Dedo J, Ayté J, Hidalgo E. Genome-wide screening of regulators of catalase expression: role of a transcription complex and histone and tRNA modification complexes on adaptation to stress. J Biol Chem. 2016;291(2):790-9. DOI: 10.1074/jbc.M115.6966580021-9258http://hdl.handle.net/10230/25571In response to environmental cues, the mitogen-activated protein kinase Sty1-driven signaling cascade activates hundreds of genes to induce a robust anti-stress cellular response in fission yeast. Thus, upon stress imposition Sty1 transiently accumulates in the nucleus where it up-regulates transcription through the Atf1 transcription factor. Several regulators of transcription and translation have been identified as important to mount an integral response to oxidative stress, such as the Spt-Ada-Gcn5-acetyl transferase or Elongator complexes, respectively. With the aim of identifying new regulators of this massive gene expression program, we have used a GFP-based protein reporter and screened a fission yeast deletion collection using flow cytometry. We find that the levels of catalase fused to GFP, both before and after a threat of peroxides, are altered in hundreds of strains lacking components of chromatin modifiers, transcription complexes, and modulators of translation. Thus, the transcription elongation complex Paf1, the histone methylase Set1-COMPASS, and the translation-related Trm112 dimers are all involved in full expression of Ctt1-GFP and in wild-type tolerance to peroxidesapplication/pdfeng© American Society for Biochemistry and Molecular Biology (ASBMB) /nThis research was originally published in Journal of biological chemistry. García P, Encinar Del Dedo J, Ayté J, Hidalgo E. Genome-wide screening of regulators of catalase expression: role of a transcription complex and histone and tRNA modification complexes on adaptation to stress. Journal of biological chemistry. 2016; 291(2): 790-799Histonesinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1074/jbc.M115.696658Stress responseGene expressionCatalasetRNASchizosaccharomyces pombeinfo:eu-repo/semantics/openAccess