Borràs, EvaSabidó Aguadé, Eduard, 1981-2018-11-162018-11-162018Borràs E, Sabidó E. DIA+: a data-independent acquisition method combining multiple precursor charges to improve peptide signal. Anal Chem. 2018 Nov 6; 90(21): 12339-12341. DOI: 10.1021/acs.analchem.8b034180003-2700http://hdl.handle.net/10230/35779Data-independent acquisition methods that acquire fragment ions from virtually any peptide in a sample have expanded the benefits of low-throughput targeted proteomics to proteome-wide analyses. While these methods have increased the reproducibility of peptide quantification across multiple samples, their sensitivity is still limited, and the quantification of complete proteomes remains a challenge. Here we present DIA+, a DIA method that combines signals from identical peptides with different charge states, resulting in improved signal-to-noise, additional number of fragments, and therefore in a higher number of identified and quantified peptides in complex samples.application/pdfengThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.analchem.8b03418ProteòmicaProteïnesPèptidsinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1021/acs.analchem.8b03418info:eu-repo/semantics/embargoedAccess