Zamora-Perez, PaulaTsoutsi, DionysiaXu, RuixueRivera Gil, Pilar, 1976-2019-05-032019-05-032018Zamora-Perez P, Tsoutsi D, Xu R, Rivera Gil P. Hyperspectral-enhanced dark field microscopy for single and collective nanoparticle characterization in biological environments. Materials (Basel). 2018; 11(2). pii: E243. DOI 10.3390/ma110202431996-1944http://hdl.handle.net/10230/37167We review how the hyperspectral dark field analysis gives us quantitative insights into the manner that different nanoscale materials interact with their environment and how this relationship is directly expressed in an optical readout. We engage classification tools to identify dominant spectral signatures within a scene or to qualitatively characterize nanoparticles individually or in populations based on their composition and morphology. Moreover, we follow up the morphological evolution of nanoparticles over time and in different biological environments to better understand and establish a link between the observed nanoparticles' changes and cellular behaviors.application/pdfeng© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ma11020243Single-particle trackingHyperspectral analysis of nanoparticles evolutionProtein coronaColloidal stabilityLiving organismsScatteringCorrelating physicochemical properties with biological responsesEnhanced dark field imaginginfo:eu-repo/semantics/openAccess