Begik, OguzhanMattick, John S.Novoa, Eva Maria2023-01-242023-01-242022Begik O, Mattick JS, Novoa EM. Exploring the epitranscriptome by native RNA sequencing. RNA. 2022 Nov;28(11):1430-9. DOI: 10.1261/rna.079404.1221355-8382http://hdl.handle.net/10230/55416Chemical RNA modifications, collectively referred to as the "epitranscriptome," are essential players in fine-tuning gene expression. Our ability to analyze RNA modifications has improved rapidly in recent years, largely due to the advent of high-throughput sequencing methodologies, which typically consist of coupling modification-specific reagents, such as antibodies or enzymes, to next-generation sequencing. Recently, it also became possible to map RNA modifications directly by sequencing native RNAs using nanopore technologies, which has been applied for the detection of a number of RNA modifications, such as N6-methyladenosine (m6A), pseudouridine (Ψ), and inosine (I). However, the signal modulations caused by most RNA modifications are yet to be determined. A global effort is needed to determine the signatures of the full range of RNA modifications to avoid the technical biases that have so far limited our understanding of the epitranscriptome.application/pdfeng© 2022 Begik et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article, published in RNA, is available undera Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1261/rna.079404.122RNA modificationsEpitranscriptomicsNanopore sequencingNative RNA sequencinginfo:eu-repo/semantics/openAccess