Castellá, MoisésBlasco-Roset, AlbertPeyrou, MarionGavaldà-Navarro, AleixVillarroya, JoanQuesada-López, TaniaLorente-Poch, LeyreSancho Insenser, JuanSzymczak, FlorianPiron, AnthonyRodríguez-Fernández, SoniaCarobbio, StefaniaGoday Arno, AlbertoDomingo, PereVidal-Puig, AntonioGiralt, MartaEizirik, Décio L.Villarroya, FrancescCereijo, Rubén2024-03-082024-03-082023Castellá M, Blasco-Roset A, Peyrou M, Gavaldà-Navarro A, Villarroya J, Quesada-López T, Lorente-Poch L, Sancho J, Szymczak F, Piron A, Rodríguez-Fernández S, Carobbio S, Goday A, Domingo P, Vidal-Puig A, Giralt M, Eizirik DL, Villarroya F, Cereijo R. Adipose tissue plasticity in pheochromocytoma patients suggests a role of the splicing machinery in human adipose browning. iScience. 2023 May 9;26(6):106847. DOI: 10.1016/j.isci.2023.1068472589-0042http://hdl.handle.net/10230/59354Adipose tissue from pheochromocytoma patients acquires brown fat features, making it a valuable model for studying the mechanisms that control thermogenic adipose plasticity in humans. Transcriptomic analyses revealed a massive downregulation of splicing machinery components and splicing regulatory factors in browned adipose tissue from patients, with upregulation of a few genes encoding RNA-binding proteins potentially involved in splicing regulation. These changes were also observed in cell culture models of human brown adipocyte differentiation, confirming a potential involvement of splicing in the cell-autonomous control of adipose browning. The coordinated changes in splicing are associated with a profound modification in the expression levels of splicing-driven transcript isoforms for genes involved in the specialized metabolism of brown adipocytes and those encoding master transcriptional regulators of adipose browning. Splicing control appears to be a relevant component of the coordinated gene expression changes that allow human adipose tissue to acquire a brown phenotype.application/pdfeng© 2023 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.isci.2023.106847Biopsy sampleSpecialized functions of cellsTranscriptomicsinfo:eu-repo/semantics/openAccess