Chiva, CristinaOlivella, RogerStaes, AnMendes Maja, TeresaPanse, ChristianStejskal, KarelDouché, ThibautLombard, BérangèreSchuhmann, AndreaLoew, DamarysMechtler, KarlMatondo, MarietteRettel, MandyHelm, DominicImpens, FrancisDevos, SimonShevchenko, AnnaNanni, PaoloSabidó Aguadé, Eduard, 1981-2025-03-252025-03-252025Chiva C, Olivella R, Staes A, Mendes Maia T, Panse C, Stejskal K, et al. A multiyear longitudinal harmonization study of quality controls in mass spectrometry proteomics core facilities. J Proteome Res. 2025 Feb 7;24(2):397-409. DOI: 10.1021/acs.jproteome.4c003591535-3893http://hdl.handle.net/10230/70002Quality control procedures play a pivotal role in ensuring the reliability and consistency of data generated in mass spectrometry-based proteomics laboratories. However, the lack of standardized quality control practices across laboratories poses challenges for data comparability and reproducibility. In response, we conducted a harmonization study within proteomics laboratories of the Core for Life alliance with the aim of establishing a common quality control framework, which facilitates comprehensive quality assessment and identification of potential sources of performance drift. Through collaborative efforts, we developed a consensus quality control standard for longitudinal assessment and adopted common processing software. We generated a 4-year longitudinal data set from multiple instruments and laboratories, which enabled us to assess intra- and interlaboratory variability, to identify causes of performance drift, and to establish community reference values for several quality control parameters. Our study enhances data comparability and reliability and fosters a culture of collaboration and continuous improvement within the proteomics community to ensure the integrity of proteomics data.application/pdfeng© 2025 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY-NC-ND 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.1021/acs.jproteome.4c00359Liquid chromatographyLongitudinal studyMass spectrometryProteomicsQuality controlinfo:eu-repo/semantics/openAccess