Puighermanal Puigvert, Emma, 1983-Castell, LaiaEsteve-Codina, AnnaMelser, SuKaganovsky, KonstantinZussy, CharleineBoubaker-Vitre, JihaneGut, MartaRialle, StephanieKellendonk, ChristophSanz, ElisendaQuintana, AlbertMarsicano, GiovanniMartin, MiquelRubinstein, MarceloGirault, Jean-AntoineDing, Jun B.Valjent, Emmanuel2020-05-122020-05-122020Puighermanal E, Castell L, Esteve-Codina A, Melser S, Kaganovsky K, Zussy C, Boubaker-Vitre J, Gut M, Rialle S, Kellendonk C, Sanz E, Quintana A, Marsicano G, Martin M, Rubinstein M, Girault JA, Ding JB, Valjent E. Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatum. Nat Commun. 2020; 11(1):1957. DOI: 10.1038/s41467-020-15716-92041-1723http://hdl.handle.net/10230/44492Action control is a key brain function determining the survival of animals in their environment. In mammals, neurons expressing dopamine D2 receptors (D2R) in the dorsal striatum (DS) and the nucleus accumbens (Acb) jointly but differentially contribute to the fine regulation of movement. However, their region-specific molecular features are presently unknown. By combining RNAseq of striatal D2R neurons and histological analyses, we identified hundreds of novel region-specific molecular markers, which may serve as tools to target selective subpopulations. As a proof of concept, we characterized the molecular identity of a subcircuit defined by WFS1 neurons and evaluated multiple behavioral tasks after its temporally-controlled deletion of D2R. Consequently, conditional D2R knockout mice displayed a significant reduction in digging behavior and an exacerbated hyperlocomotor response to amphetamine. Thus, targeted molecular analyses reveal an unforeseen heterogeneity in D2R-expressing striatal neuronal populations, underlying specific D2R's functional features in the control of specific motor behaviors.application/pdfeng© The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Functional and molecular heterogeneity of D2R neurons along dorsal ventral axis in the striatuminfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41467-020-15716-9Basal gangliaMolecular neuroscienceinfo:eu-repo/semantics/openAccess