Heesters, Balthasar A.van Megesen, KyahTomris, Ilhande Vries, Robert P.Magri, Giuliana, 1978-Spits, Hergen2022-06-222022-06-222021Heesters BA, van Megesen K, Tomris I, de Vries RP, Magri G, Spits H. Characterization of human FDCs reveals regulation of T cells and antigen presentation to B cells. J Exp Med. 2021 Oct 4; 218(10): e20210790. DOI: 10.1084/jem.20210790.0022-1007http://hdl.handle.net/10230/53560Stromal-derived follicular dendritic cells (FDCs) are essential for germinal centers (GCs), the site where B cells maturate their antibodies. FDCs present native antigen to B cells and maintain a CXCL13 gradient to form the B cell follicle. Yet despite their essential role, the transcriptome of human FDCs remains undefined. Using single-cell RNA sequencing and microarray, we provided the transcriptome of these enigmatic cells as a comprehensive resource. Key genes were validated by flow cytometry and microscopy. Surprisingly, marginal reticular cells (MRCs) rather than FDCs expressed B cell activating factor (BAFF). Furthermore, we found that human FDCs expressed TLR4 and can alter antigen availability in response to pathogen-associated molecular patterns (PAMPs). High expression of PD-L1 and PD-L2 on FDCs activated PD1 on T cells. In addition, we found expression of genes related to T cell regulation, such as HLA-DRA, CD40, and others. These data suggest intimate contact between human FDCs and T cells.application/pdfeng© 2021 Heesters et al. This article is available under a Creative Commons License (Attribution 4.0 International, as described at http://creativecommons.org/licenses/by/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.1084/jem.20210790Cèl·lules dendrítiquesCèl·lules TCèl·lules Binfo:eu-repo/semantics/openAccess