Pekmezovic, MarinaHovhannisyan, Hrant, 1992-Gresnigt, Mark S.Iracane, EliseOliveira Pacheco, JoãoSiscar Lewin, SofíaSeemann, EricQualmann, BrittaKalkreuter, TillMüller, SylviaKamradt, ThomasMogavero, SeleneBrunke, SaschaButler, GeraldineGabaldón Estevan, Juan Antonio, 1973-Hube, Bernhard2021-07-152021Pekmezovic M, Hovhannisyan H, Gresnigt MS, Iracane E, Oliveira-Pacheco J, Siscar-Lewin S, Seemann E, Qualmann B, Kalkreuter T, Müller S, Kamradt T, Mogavero S, Brunke S, Butler G, Gabaldón T, Hube B. Candida pathogens induce protective mitochondria-associated type I interferon signalling and a damage-driven response in vaginal epithelial cells. Nat Microbiol. 2021;6(5):643-57. DOI: 10.1038/s41564-021-00875-22058-5276http://hdl.handle.net/10230/48185Vaginal candidiasis is an extremely common disease predominantly caused by four phylogenetically diverse species: Candida albicans; Candida glabrata; Candida parapsilosis; and Candida tropicalis. Using a time course infection model of vaginal epithelial cells and dual RNA sequencing, we show that these species exhibit distinct pathogenicity patterns, which are defined by highly species-specific transcriptional profiles during infection of vaginal epithelial cells. In contrast, host cells exhibit a homogeneous response to all species at the early stages of infection, which is characterized by sublethal mitochondrial signalling inducing a protective type I interferon response. At the later stages, the transcriptional response of the host diverges in a species-dependent manner. This divergence is primarily driven by the extent of epithelial damage elicited by species-specific mechanisms, such as secretion of the toxin candidalysin by C. albicans. Our results uncover a dynamic, biphasic response of vaginal epithelial cells to Candida species, which is characterized by protective mitochondria-associated type I interferon signalling and a species-specific damage-driven response.application/pdfeng© Springer Nature Publishing AG Pekmezovic M, Hovhannisyan H, Gresnigt MS, Iracane E, Oliveira-Pacheco J, Siscar-Lewin S, Seemann E, Qualmann B, Kalkreuter T, Müller S, Kamradt T, Mogavero S, Brunke S, Butler G, Gabaldón T, Hube B. Candida pathogens induce protective mitochondria-associated type I interferon signalling and a damage-driven response in vaginal epithelial cells. Nat Microbiol. 2021;6(5):643-57. DOI: 10.1038/s41564-021-00875-2 [http://dx.doi.org/10.1038/s41564-021-00875-2]info:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41564-021-00875-2Fungal host responseFungal pathogenesisInnate immunityTranscriptomicsinfo:eu-repo/semantics/openAccess